Number of Autonomous Replication Sequence elements (ARSs) in the genome

Range ~200 - 400 ARSs/genome
Organism Budding yeast Saccharomyces cerevisiae
Reference Raghuraman et al., Replication dynamics of the yeast genome. Science. 2001 Oct 5 294(5540):115-21. DOI: 10.1126/science.294.5540.115 p.115 right columnPubMed ID11588253
Primary Source Rivin CJ, Fangman WL. Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae. J Cell Biol. 1980 Apr85(1):108-15. & C. S. Newlon, W. Burke, in ICN-UCLA Symposium on Molecular and Cellular Biology (Academic Press, New York, vol. 19, 1980), pp. 399-409.PubMed ID6767729
Method Abstract:"Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae."
Comments p.115 middle column:"The replication of eukaryotic chromosomes is highly regulated. Replication is limited to the S phase of the cell cycle and within S phase, initiation of replication is controlled with respect to both location and time. The sites of initiation, called replication origins, have been best characterized in the budding yeast Saccharomyces cerevisiae, in which a functional assay based on plasmid maintenance has allowed identification of potential origins of replication [autonomous replication sequence elements (ARSs)]. There are estimated to be ~200 to 400 ARSs in the yeast genome (primary sources), and most, but not all, function as chromosomal origins (ref 3)."
Entered by Uri M
ID 111672