Mitochondrial activities of yeast cells throughout the culture period

Range Table - link
Organism Budding yeast Saccharomyces cerevisiae
Reference Dejean L, Beauvoit B, Guérin B, Rigoulet M. Growth of the yeast Saccharomyces cerevisiae on a non-fermentable substrate: control of energetic yield by the amount of mitochondria. Biochim Biophys Acta. 2000 Feb 24 1457(1-2):45-56. p.52 table 2PubMed ID10692549
Method p.46 left column 2nd paragraph:"...[investigators] used a respirometric and on-line calorimetric approach to analyse the energetic balances and the control of energetic metabolism during growth phase transitions of batch grown S. cerevisiae with limiting amounts of a non-fermentable carbon source (lactate)."
Comments p.53 left column bottom paragraph:"The uncoupled respiration of yeast cells measured with lactate 0.2% as respiratory substrate continuously declined during the growth period (Fig. 7B). Moreover, Table 2 shows that the maximal respiratory capacity (JOmax) measured in the presence of ClCCP and saturating amounts of lactate, glucose and ethanol (0.2%, 20 mM and 100 mM in the culture medium, respectively [ref 9]) also decreased during the transition from the early to the late exponential phase, as well as to the stationary phase. In this way, this growth phase-dependent modulation of the maximal respiratory capacity of yeast cells was investigated in relation to the mitochondrial enzyme content." ClCCP=carbonyl cyanide m-chlorophenyl hydrazone. JO=Respiratory rates
Entered by Uri M
ID 111641