Metabolite production from different cultivations

Range Table - link C-mmol/C-mol substrate
Organism Yeast Kluyveromyces marxianus
Reference Fonseca G. G., Gombert A. K., Heinzle E., Wittmann C. Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source. FEMS Yeast Research, 7: 422–435, 2007 p.428 table 2PubMed ID17233766
Method p.423 right column 2nd paragraph:"With the aim of increasing [investigators'] knowledge on the macroscopic physiology of K. marxianus, [they] performed batch and continuous bioreactor cultivations of this yeast under welldefined conditions, and carried out measurements which allowed [them] quantitatively to describe growth, substrate consumption, metabolite formation, biomass composition and respiratory parameters, when glucose is used as the sole carbon source."
Comments p.425 right column 4th paragraph:"Metabolites were formed to a very low extent under the conditions employed (Tables 2 and 3), totalling always <3% of the consumed carbon, except for the chemostat at 0.5 h^-1, in which total metabolites corresponded to 6% of the consumed carbon. However, as the fed glucose was not completely consumed by the yeast cells in the latter experiment, this was not a carbon-limited culture, which is a different physiological situation as compared with the other cultures performed in this work. In general, metabolite formation increased with dilution rate. Interestingly, at D=0.1 h^-1 and 2.5 v.v.m., metabolite formation was higher than for the culture at the same dilution rate and 1v.v.m. air sparging, and also higher for some metabolites as compared with the culture at 0.25 h^-1. In general, acetate, pyruvate and 2-oxoglutarate were the organic acids present in highest concentrations (Table 2 Fig. 2)." See notes beneath table
Entered by Uri M
ID 111638