| Range |
13 - 14 domain repeats
|
| Organism |
Bacteria Escherichia coli |
| Reference |
King JD et al., Lipopolysaccharide O antigen size distribution is determined by a chain extension complex of variable stoichiometry in Escherichia coli O9a. Proc Natl Acad Sci U S A. 2014 Apr 29111(17):6407-12. doi: 10.1073/pnas.1400814111. p.6408 figure 1BPubMed ID24733938
|
| Method |
p.6408 right column 2nd paragraph:"In this study [investigators] show that
E. coli O9a OPS length distribution
can be quantitatively modulated by titrating the expression of
either of the biosynthetic enzymes, WbdD and WbdA." |
| Comments |
p.6407 left column 3rd paragraph:"Lipopolysaccharide (LPS) is a glycolipid which constitutes the
major and characteristic component of the outer leaflet of the
outer membrane of most Gram negative bacterial cell envelopes
(reviewed in ref. 1). Most bacteria produce LPS molecules that
contain a conserved lipid A moiety linked to a short (core) oligosaccharide. In many species, a proportion of LPS molecules
have an additional domain known as O polysaccharide (OPS), O
antigen or O chain. OPS is a carbohydrate polymer in which the
repeating unit (O unit) consists of a short oligosaccharide or
sometimes a single sugar. OPS is synthesized template-free by
glycosyltransferases and is found in lengths that vary from one
repeat unit to several hundred sugars." p.6408 left column 2nd paragraph:"The prototype for the ABC transporter-dependent pathway is
the OPS from
Escherichia coli
O9a. This OPS has a four-sugar
repeat unit containing
a
1
?
2, and
a
1
?
3-linked
D
-mannose
(Fig. 1
C
) (ref 10) with a unimodal chain-length distribution (Fig.
1
B
). Biosynthesis of the repeating domain of this OPS is catalyzed by WbdA, a chain extension enzyme containing two glycosyltransferase domains (ref 11)." |
| Entered by |
Uri M |
| ID |
111526 |