||"By homology, the only CLC protein of Saccharomyces cerevisiae, Gef1, belongs to this family of intracellular exchangers." "Expression of any Gef1 variant resulted in a cytosolic pH
close to 6 (differences to the deletion strain are statistically
<0.02). At the same time, the Gef1-containing compartment was slightly acidic with respect to the cytosol and the
vacuolar lumen substantially more acidic (Fig. 5B,C,D,E), as
expected for these acidifying compartments from the literature
(Klionsky et al., 1992, Manolson et al., 1994, Martinez-Munoz
and Kane, 2008, Plant et al., 1999, Rothman et al., 1989)."