Measured elemental composition (% of dry weight) of E. coli biomass at different dilution rates (D) and glucose concentrations in the feed (CS,in)

Range Table - link
Organism Bacteria Escherichia coli
Reference Taymaz-Nikerel H, Borujeni AE, Verheijen PJ, Heijnen JJ, van Gulik WM. Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng. 2010 Oct 1 107(2):369-81. doi: 10.1002/bit.22802. p.375 table IVPubMed ID20506321
Method Abstract:"The unknown ATP stoichiometry parameters of the constructed E. coli network were estimated from experimental data of eight different aerobic chemostat experiments carried out with E. coli MG1655, grown at different dilution rates (0.025, 0.05, 0.1, and 0.3 h(-1)) and on different carbon substrates (glucose, glycerol, and acetate)."
Comments "The elemental compositions of freeze dried biomass samples from the different aerobic glucose-limited cultures were analyzed with respect to their C, H, O, N, and S content. The results showed (Table IV) that the elemental compositions were similar for the different growth rates and substrate concentrations in the feed. The carbon and nitrogen contents appeared comparable with published values for aerobic growth on glucose (Bratbak and Dundas, 1984 Han et al., 2003 Heldal et al., 1985)." "From the obtained biochemical composition the average elemental composition was calculated as CH1.63N0.25 O0.38S0.006, with generalized degree of reduction ?x=4.18. The calculated elemental composition appeared to be significantly different from the average measured elemental composition which was calculated from the data presented in Table IV as CH2.01N0.23O0.48S0.004, with ?x=4.37." Title of table:"Measured elemental composition (% of dry weight) of E. coli biomass at different dilution rates (D) and glucose concentrations in the feed (CS,in)" For conditions of experiments see table I beneath table IV.
Entered by Uri M
ID 111459