Mean mCherry maturation time

Value 69 min Range: 45 - 114 min
Organism Bacteria Escherichia coli
Reference Hebisch E, Knebel J, Landsberg J, Frey E, Leisner M. High variation of fluorescence protein maturation times in closely related Escherichia coli strains. PLoS One. 2013 Oct 14 8(10):e75991. doi: 10.1371/journal.pone.0075991. p.6 right column top paragraphPubMed ID24155882
Method "Here, [investigators] analyzed the maturation kinetics of the fluorescent protein mCherry. As a derivative of DsRed [ref 18 PMID 15558047], mCherry chromophore formation has to undergo two subsequent oxidation steps [ref 29 PMID 16893165]. The first oxidation step creates a green intermediate [ref 28 PMID 10504696], and the second oxidation step leads to an increase in conjugated double bonds. As a consequence, one observes a shift to longer absorbance and emission wavelengths [ref 28 PMID 10504696]. [Investigators] measured both the temporal fluorescence development of the green intermediate and of the red fluorescent state of the final mCherry protein in parallel (Figure S5). [They] obtained the characteristic sigmoidal fluorescence development for the red fluorescent protein after CAP addition."
Comments "Whereas maturation kinetics of the mCherry ancestor DsRed was theoretically described by a threestep process [ref 25 PMID 11812239], [investigators’] experimentally observed data for mCherry maturation kinetic was adequately described by a two-step process with an additional back reaction from the red fluorescent state to the green fluorescent state (Supporting Information S1). The average mCherry maturation time of the S, R and C strain was 69 min, with a high variation ranging from 45 to 114 min. [Investigators’] three E. coli strains showed significantly longer mCherry maturation times than for example E. coli LMG194 with 15 min [ref 18 PMID 15558047] or PAmCherrys expressed in E. coli LMG194 with 18 to 25 min [ref 19 PMID 19169259]."
Entered by Uri M
ID 111423