Ca2+ concentration

Range ~90 nM
Organism Bacteria Escherichia coli
Reference Booth IR, Miller S, Müller A, Lehtovirta-Morley L. The evolution of bacterial mechanosensitive channels. Cell Calcium. 2015 Mar57(3):140-50. doi: 10.1016/j.ceca.2014.12.011. p.142 left column 2nd paragraphPubMed ID25591932
Primary Source P. Gangola, B.P. Rosen, Maintenance of intracellular calcium in Escherichia coli, J. Biol. Chem. 262 (1987) 12570–12574.PubMed ID2442165
Method "Cells of E. coli were loaded with the membrane-permeable acetoxymethyl ester of fura-2, which was cleaved intracellularly to give the free pentaacid."
Comments "Bacterial Ca2+ pools of around 90 nM have been measured in cells incubated with mM external Ca2+ [primary source]. Selective exclusion of Ca2+ must have arisen early in evolution, given that it is desirable not to allow its penetration into the cytoplasm." Primary source abstract: "Recently a series of fluorescent calcium indicator dyes have been developed for measurement of free intracellular calcium in eukaryotic cells. Here [researchers] report the use of one such dye, fura-2, for the study of intracellular calcium levels in the prokaryote Escherichia coli. Cells of E. coli were loaded with the membrane-permeable acetoxymethyl ester of fura-2, which was cleaved intracellularly to give the free pentaacid. The concentration of free [Ca2+]i in unstarved cells was maintained at 90 +/- 10 nM, irrespective of the Ca2+ concentration in the extracellular medium."
Entered by Uri M
ID 111366