| Range |
Table - link
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Soboll S, Scholz R, Heldt HW. Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of perfused rat liver. Eur J Biochem. 1978 Jun 15 87(2):377-90. p.382 table 3PubMed ID668699
|
| Method |
“From marker enzyme activities and metabolite
contents in the unfractionated homogenate and in
the different fractions the mitochondrial and cytosolic
metabolite contents (nmol/mg protein) were calculated
by extrapolating the data to fractions of pure mitochondria
and pure cytosol according to Elbers et al.
[ref 5]. The subcellular metabolite contents were converted
into concentrations assuming a water content of 0.8 and 3.8 µl/mg protein in the mitochondrial and
cytosolic compartment, respectively, based on considerations
previously discussed by Soboll et al. [ref 1].” |
| Comments |
"Under conditions of substrate-free perfusion, the main substrates for energy yielding metabolism are endogenous fatty acids, since high rates of ketogenesis were observed (Table 3, expt H). When suitable substrates are added, such as lactate (Fig. 1) or dihydroxyacetone, the livers synthesize glucose at high rates, whereas ketogenesis is suppressed. Glycolysis is minimal in livers from starved rats. Production of lactate + pyruvate from exogenous glucose is observed at considerable rates only when glucose is present in high concentrations (Table 3, expt H-G)." For experimental conditions and metabolic states see p.381 table 1 link |
| Entered by |
Uri M |
| ID |
111355 |