Phenomic and modeling data from growth screens of G. metallireducens GS-15 wild type and dcuB with acetate as an electron donor

Range Table - link
Organism Bacteria Geobacter metallireducens
Reference Feist AM. et al., Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. PLoS Comput Biol. 2014 Apr 24 10(4):e1003575. doi: 10.1371/journal.pcbi.1003575. p.7 table 3PubMed ID24762737
Method "An updated reconstruction of G. metallireducens GS-15, iAF987, was generated by reconciling an existing genome-scale reconstruction [13] and an updated genome annotation, performing a bottomup reconstruction of additional metabolic pathways."
Comments "The first step in the modeling process was to examine the fumarate reductase reaction and maintenance energies necessary for predicting phenotypes using constraint-based analysis (Step 1, Figure 3C). To examine this content, data was generated using the G. metallireducens dcuB strain and utilized in simulations. An electrogenic bifunctional fumarate reductase/succinate dehydrogenase (FRD2rpp) was included based on recent biochemical evidence in a similar species [15]. This inclusion was validated with the growth data of G. metallireducens on acetate and fumarate using the dcuB strain. The earlier version of the G. metallireducens model [13] and the current model (iAF987) without an electrogenic fumarate reductase were unable to produce a feasible solution when constrained with experimentally measured acetate and fumarate uptake rates (see Table 3)."
Entered by Uri M
ID 111307