Transcription and translation accuracy

Range Transcription 1:60,000 - 1:120,000 wrong/correct nucleotides: translation ≥1:3,000 wrong/correct aa
Organism Bacteria Escherichia coli
Reference Ulrich Stelzl, Sean Connell, Knud H Nierhaus, Brigitte Wittmann-Liebold, Ribosomal Proteins: Role in Ribosomal Functions, 2001, DOI: 10.1038/npg.els.0000687, p.1 left column bottom paragraph and right column top paragraph
Comments p.1 left column bottom paragraph and right column top paragraph:"Ribosomes consist of a small number (3 or 4) of large rRNA molecules (5S–28S rRNA with 120 to about 4500 nucleotides) and a large number (50–80) of small proteins (r-proteins with typically 25–300 amino acids). The fact that ribosomal proteins are short in comparison to the long rRNA molecules is thought to be related to the high accuracy of transcription compared to translation. The transcription accuracy is in the order of 1:60 000 to 1:120 000 (one wrong nucleotide per 120 000 incorporated nucleotides) and this allows the synthesis of accurate rRNAs of 3000 to 5000 nucleotides without problems, whereas the translational accuracy is not better than 1:3000. Thus proteins should be composed of fewer amino acids than rRNA of nucleotides in order to allow the synthesis of accurate ribosomal proteins. During formation of functional ribosomes, correctly synthesized r-proteins are selected by the assembly process." Corresponding to transcriptional error rate of 8.3e-6/nuc - 1.7e-5/nuc, translational error rate of =3.3e-4/aa [aa=amino acid]
Entered by Uri M
ID 111267