Cell dry mass in two TEM-based studies

Range exponentially growing cells 489fg and 710fg: stationary cells 179 fg and 180 fg fg
Organism Bacteria Escherichia coli
Reference Feijó Delgado et al., Intracellular water exchange for measuring the dry mass, water mass and changes in chemical composition of living cells. PLoS One. 2013 Jul 2 8(7):e67590. doi: 10.1371/journal.pone.0067590 p.5 right column top paragraphPubMed ID23844039
Primary Source [5] Loferer-Kro¨ßsbacher M, Klima J, Psenner R (1998) Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Appl Environ Microbiol 64: 688–694. [7] Fagerbakke KM, Heldal M, Norland S (1996) Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria. Aquat Microb Ecol 10: 15–27. doi:10.3354/ame010015.PubMed ID9464409
Method Transmission electron microscopy
Comments "[Researchers'] dry mass results [203fg BNID 111210] are consistent with previous reported measurements of the described single-cell or bulk methods. For instance, two TEM-based studies found median E. coli dry masses of 489 fg and 710 fg for exponentially growing cells and 179 fg and 180 fg for stationary ones [primary sources]. [Researchers] report median masses of 725 fg and 179 fg, respectively, pooling technical replicates shown in Figure 2."
Entered by Uri M
ID 111211