RNA amount in cell

Range 10 - 20 pg/cell
Organism Mammals
Reference Han F, Lillard SJ. In-situ sampling and separation of RNA from individual mammalian cells. Anal Chem. 2000 Sep 1 72(17):4073-9. p.4076 right column 2nd paragraphPubMed ID10994967
Primary Source [24] Ogura et al., RNA chip: quality assessment of RNA by microchannel linear gel electrophoresis in injection-molded plastic chips. Clin Chem. 1998 Nov44(11):2249-55. [44] Kawasaki, E. S. Wang, A. M. In PCR Technology: Principles and Applications for DNA Amplification Erlich, H. A., Ed. Stockton Press: New York, 1989 p 92.PubMed ID9799750
Comments "...the expected amount of total RNA in a single cell can be estimated from the literature. From studies utilizing isolated total RNA, it is estimated that 1 µg results from (5×10^4)-(1×10^5) mammalian cells, which corresponds to 10-20 pg of RNA/cell [primary sources]."
Entered by Uri M
ID 111204