| Range |
400-900 nm
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Takeshige, Kazuhiko, et al. "Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction." The Journal of cell biology 119.2 (1992): 301-311 abstractPubMed ID1400575
|
| Method |
Abstract: "For determination of the physiological role and mechanism of vacuolar proteolysis in the yeast Saccharomyces cerevisiae, mutant cells lacking proteinase A, B, and carboxypeptidase Y were transferred from a nutrient medium to a synthetic medium devoid of various nutrients and morphological changes of their vacuoles were investigated. After incubation for 1 h in nutrient-deficient media, a few spherical bodies appeared in the vacuoles and moved actively by Brownian movement. These bodies gradually increased in number and after 3 h they filled the vacuoles almost completely. During their accumulation, the volume of the vacuolar compartment also increased. Electron microscopic examination showed that these bodies were surrounded by a unit membrane which appeared thinner than any other intracellular membrane." |
| Comments |
Abstract: "The diameter of the bodies ranged from 400 to 900 nm." |
| Entered by |
yuvgilad |
| ID |
111180 |