Fraction of transcription in HeLa cell

Range nucleolar RNA ~20%: nucleoplasmic RNA 80% %
Organism Human Homo sapiens
Reference Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 right column 3rd paragraphPubMed ID10657981
Primary Source Soeiro, R., Vaughan, M. H., Warner, J. R., and Darnell, J. E., Jr. (1968) The turnover of nuclear DNA-like RNA in HeLa cells. J. Cell Biol. 39, 112–118PubMed ID5678443
Method "...analysis performed on HeLa cells, labeled for 10 min with orthophosphate-32P"
Comments "A complementary analysis performed on HeLa cells, labeled for 10 min with orthophosphate-32P, confirmed that nucleolar and nucleoplasmic RNA accounts for ~20 and 80% of synthesis, respectively (primary source). However, in view of their unusually high level of nRNA turnover (31), it is worth considering if cells adapted for continuous culture have developed atypical patterns of RNA metabolism."
Entered by Uri M
ID 111163