Transcription rate of rRNA

Range ~2.5 kbp/min
Organism vertebrates
Reference Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 left column 2nd paragraphPubMed ID10657981
Primary Source [21] Greenberg, H., and Penman, S. (1966) Methylation and processing of ribosomal RNA in HeLa cells. J. Mol. Biol. 21, 527–535 [22] Emerson, C. P., Jr. (1971) Regulation of the synthesis and the stability of ribosomal RNA during contact inhibition of growth. Nature (London) New Biol. 232, 101–106PubMed ID19768869, 5284943
Comments P.243 left column 2nd paragraph: "In a HeLa cell, 120–150 active rRNA genes (ref 20 Jackson et al., 1998 PMID 9614191) are each associated with 100–120 transcription complexes (ref 19 Miller et al. 1972 PMID 4562652). With a synthetic rate of ~2.5 kbp/min (primary sources 21, 22), this is sufficient to yield 4×10^6 complete transcripts/22h cell cycle and maintain the observed steady-state level of ~3.5×10^6 ribosomes in these proliferative cells (ref 23)." Primary sources 21 & 22 study HeLa cells & chick skin fibroblasts, respectively.
Entered by Uri M
ID 111155