Measurement of PSII centers using change of A320

Range Table - link
Organism Chlamydomonas reinhardtii
Reference Wykoff DD, Davies JP, Melis A, Grossman AR. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol. 1998 May117(1):129-39. p.133 table IVPubMed ID9576782
Comments "The decreased maximal electron flow through PSII is a consequence of at least two processes. First, between 20 and 30% of the PSII reaction centers appear to be inactivated because of photodamage. Quantitation of photodamage is complicated by the inherent inaccuracies in quantitating protein levels by western analysis and the potential contribution of PSII QB-nonreducing centers to the loss of Fv (which can result from an apparent increase in the F0 value). However, analyses of fluorescence using a very low-intensity measuring beam (<0.01µmol photons/m^2/sec) had little effect on either F0 or Fv/Fmax (<5%), and the measurements of QA levels (Table IV) demonstrated an absolute loss of functional PSII (by approximately 30%). These results indicate that nutrient limitation results in a loss of active PSII centers." TAP=Trisacetate-P
Entered by Uri M
ID 111120