| Method |
"Cells were counted using a hemacytometer and the viability
of the cells was assessed by viability staining (Davies
et al., 1996). For chlorophyll determinations cells were
suspended in 0.01% Tween 20 (United States Biochemical)
and pelleted by centrifugation for 30 s at 16,000g. The cell
pellet was extracted in 90% acetone, cellular debris were
removed by centrifugation (30 s at 16,000g), and the chlorophyll
a and b levels were determined spectrophotometrically
(Jeffrey and Humphrey, 1975)." |