Growth of E. coli and 14CO2 evolution during incubation with [U-14C]carbon source, each at 1 mM

Range Table - link
Organism Bacteria Escherichia coli
Reference Shearer G, Lee JC, Koo JA, Kohl DH. Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli. FEBS J. 2005 Jul272(13):3260-9. p.3262 table 1PubMed ID15978033
Method "In this study, [researchers] describe experiments aimed at calculating the fraction of the flux from early glycolytic intermediates to CO2 in intact Escherichia coli. Cells incubated with [14C]glucose made 14C-labeled glycolytic intermediates. When the incubation mix also included a [12C]intermediate, assuming that this intermediate entered the cell, there was a competition between the intermediate just produced in the pathway and the same molecular species in the aqueous cytosol. To the degree the latter was successful, the amount of 14CO2 evolved was decreased. Cells were also incubated in [12C]glucose plus [14C]intermediate, [14C]glucose alone and [14C]intermediate alone. Combining these data allowed [them] to calculate the fraction of the total flux to CO2 that was channeled. [They] used an E. coli mutant that was engineered to take up 6-carbon sugar phosphates constitutively."
Comments "The cells grew well on all carbon sources of interest to [researchers], including the 6-carbon sugar phosphates, Glu6P, Fru6P, and Fru1,6P2. The doubling times for Glu6P and Fru6P were comparable with the doubling time of the parental strain growing on glucose (Table 1). Doubling times when grown on glycerol and Fru1,6P2 were considerably longer."
Entered by Uri M
ID 110836