Fraction of fructose 1,6-bisphosphate that is channelled to CO2

Value 99 % Range: ±16 %
Organism Bacteria Escherichia coli
Reference Shearer G, Lee JC, Koo JA, Kohl DH. Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli. FEBS J. 2005 Jul272(13):3260-9. p.3262 left column top paragraphPubMed ID15978033
Method "In this study, [researchers] describe experiments aimed at calculating the fraction of the flux from early glycolytic intermediates to CO2 in intact Escherichia coli. Cells incubated with [14C]glucose made 14C-labeled glycolytic intermediates. When the incubation mix also included a [12C]intermediate, assuming that this intermediate entered the cell, there was a competition between the intermediate just produced in the pathway and the same molecular species in the aqueous cytosol. To the degree the latter was successful, the amount of 14CO2 evolved was decreased. Cells were also incubated in [12C]glucose plus [14C]intermediate, [14C]glucose alone and [14C]intermediate alone. Combining these data allowed [them] to calculate the fraction of the total flux to CO2 that was channeled. [They] used an E. coli mutant that was engineered to take up 6-carbon sugar phosphates constitutively."
Comments "The results indicate a high degree of channeling of fructose 1,6-bisphosphate (Fru1,6P2) to CO2 (99±16% of the flux from Fru1,6P2 channeled, P=0.005). This result requires that all of the downstream steps from Fru1,6P2 to pyruvate and the further oxidation of pyruvate to CO2 (presumably via mixed acid fermentation) be 100% channeled."
Entered by Uri M
ID 110834