3HHO (tritiated water) permeability of lipid bilayer membranes made from red cells

Value 3.2e-4 cm/sec Range: ±0.1e-4 cm/sec
Organism Human Homo sapiens
Reference Wood RE, Wirth FP Jr, Morgan HE. Glucose permeability of lipid bilayer membranes. Biochim Biophys Acta. 1968 Sep 17 163(2):171-8. p.175 2nd paragraphPubMed ID5686274
Method "Bimolecular lipid membranes have been prepared from total phospholipid extracts of human red cells. The electrical properties and water permeability of these membranes were similar to values reported earlier for other bilayer membranes...Methods for the determination of permeability coefficients (k) of radioactive solutes were developed with the use of 3HHO as the penetrating species."
Comments "The permeability coefficient of 3HHO k[3HHO] was 3.2X10^-4±0.1X10^-4cm/sec (14 membranes) in close agreement with the values reported by others [refs 8,10,16]. In these experiments there was no provision for stirring the fluid in the chambers beyond initial mixing and the currents generated in the recipient chamber by the flow of perfusate. Donor chamber concentrations of 3HHO were usually about 2X10^6 counts/min per ml."
Entered by Uri M
ID 110833