Urea permeability in red cells at 25°C and pH 7.2

Range Table - link
Organism Human Homo sapiens
Reference Brahm J. Urea permeability of human red cells. J Gen Physiol. 1983 Jul82(1):1-23. p.10 table IIPubMed ID6411854
Method "The rate of unidirectional [14C]urea efflux from human red cells was determined in the self-exchange and net efflux modes with the continuous flow tube method."
Comments p.9 bottom line:"The effects of phloretin, the sulfhydryl (SH)-specific reagents PCMBS [p-chloromercuribenzosulfonate], NEM [N-ethyl-maleimide], and DTNB [5,5'-dithiobis-2-nitrobenzoate], and the anion transport inhibitor DIDS [4,4'-diisothiocyano-2,2'-stilbenedisulfonate] are shown in Table II." p.17 2nd paragraph:"The concentration-independent residual urea permeability after phloretin inhibition was low (Table II) and possessed a high EA of59 kJ/mol, in conformity with properties of the phloretin-insensitive urea permeation through the lipid core of the membrane in red cells from duck (J. O. Wieth and J. Brahm, unpublished results) and chicken (Brahm and Wieth, 1977)." See note beneath table
Entered by Uri M
ID 110729