Distribution of 13C in HeLa cells labeled with 5% [U-13C]glucose for 48 h

Range Table - link % of glucose-derived carbon in biomass
Organism Human Homo sapiens
Reference Chen YJ, Huang X, Mahieu NG, Cho K, Schaefer J, Patti GJ. Differential Incorporation of Glucose into Biomass during Warburg Metabolism. Biochemistry. 2014 Jul 29 53(29):4755-7. doi: 10.1021/bi500763u. p.4756 table 1PubMed ID25010499
Method "[Researchers'] approach to studying the fate of glucose was to integrate two complementary metabolomic technologies: solid-state nuclear magnetic resonance (NMR) and liquid chromatography/mass spectrometry (LC/MS)."
Comments "By monitoring changes in the integrated intensity of the 13C3 peak of lactate (22 ppm), [researchers] determined that 76% of labeled glucose carbon is secreted as lactate. The combination of multiple NMR experiments allowed the quantitation of the fate of labeled glucose carbon in proteins, peptides, intracellular sugars and glycerol, and lipids, yielding values of 9, 2, 7, and 4%, respectively (Table 1)."
Entered by Uri M
ID 110719