Viral reads classification

Range Table - link
Organism Virus
Reference Popgeorgiev N et al., Marseillevirus-like virus recovered from blood donated by asymptomatic humans. J Infect Dis. 2013 Oct 1 208(7):1042-50. doi: 10.1093/infdis/jit292.PubMed ID23821720
Method "Pyrosequencing DNA extracted from the viral-enriched fraction of the blood of asymptomatic blood donors generated 20,238 reads (NCBI accession number: PRJNA183996), which were classified by their taxonomic distribution (Figure 1). A best BLAST hits comparison against the GenBank database (BLASTn E value, <10^-5) indicated that 17,257 metagenomic reads (85.3%) had significant hits and that 67.7% of these reads were related to viruses."
Comments "The majority of these sequences [see Measurement Method] aligned with viruses from the Anelloviridae family, including Torque Teno virus (TTV), TTV-like virus, SEN virus, TTV midi virus, and TTV-like mini virus (Table 1), which has been previously reported [5, 12, 16]. However, [researchers] unexpectedly found that 2.5% of the viral metagenome matched that of Marseillevirus. [They] were initially unconvinced that these data were correct, as this virus has only been found in [their] laboratory."
Entered by Uri M
ID 110702