iBMK cell contents

Range DNA 11: RNA 14: protein 88 µg/µl cells
Organism Mammalian tissue culture cell
Reference Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. p.8 right column 3rd paragraphPubMed ID24301801
Method "Specifically, [researchers] measured the DNA, RNA, and protein contents to be equal to 11, 14, and 88 µg/µl cells, respectively, in iBMK cells. Cellular demands for acetyl-CoA based on steady-state fatty acid concentrations, cellular growth rate, and fatty acid uptake rates are shown in Supplementary Table 5 [BNID 110690] and (v) measured flux in the serine biosynthesis pathway."
Comments iBMK=Immortalized baby mouse kidney epithelial cells
Entered by Uri M
ID 110691