Relative contribution of oxidative phosphorylation and aerobic glycolysis to ATP production

Range oxidative phosphorylation ~80%: aerobic glycolysis ~20% %
Organism Mammalian tissue culture cell
Reference Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, Rabinowitz JD. Glutamine-driven oxidative phosphorylation is a major ATP source in transformed mammalian cells in both normoxia and hypoxia. Mol Syst Biol. 2013 Dec 3 9: 712. doi: 10.1038/msb.2013.65. p.3 left columnPubMed ID24301801
Method "To study ATP production routes, [researchers] used Bax-/-, Bak-/- murine renal epithelial cells immortalized by expression of adenovirus E1A and dominant-negative p53 (Degenhardt et al, 2002) (iBMK cells)."
Comments "The total ATP production rate in the parental iBMK cell line was found to be 861 nmole/µl cells/h (where µl refers to the packed cell volume, with 1µl equal to ~10^6 cells). The relative contribution of oxidative phosphorylation and aerobic glycolysis was ~80 and 20%, respectively (Supplementary Table 3 BNID 110689), consistent with previous results (Zu and Guppy, 2004, PMID 14697210)." iBMK=Immortalized baby mouse kidney epithelial cells
Entered by Uri M
ID 110684