Protein degradation rates (most stable proteins)

Range phosphoglycerate kinase 0.056 enolase 0.049 1/hour
Organism Bacteria Streptomyces coelicolor
Reference Trötschel C, Albaum SP, Poetsch A. Proteome turnover in bacteria: current status for Corynebacterium glutamicum and related bacteria. Microb Biotechnol. 2013 Nov6(6):708-19. doi: 10.1111/1751-7915.12035 p.712 right column top paragraphPubMed ID23425033
Primary Source Jayapal, K.P., Sui, S., Philp, R.J., Kok, Y.J., Yap, M.G., Griffin, T.J., and Hu, W.S. (2010) Multitagging proteomic strategy to estimate protein turnover rates in dynamic systems. J Proteome Res 9: 2087–2097.PubMed ID20184388
Method "In an effort to segregate these two underlying mechanisms [protein synthesis and breakdown], a novel multitagging approach was developed based on stable isotope tagging (SILAC) and isobaric tag for relative and absolute quantification (iTRAQ) labelling (primary source)."
Comments "Using this approach, protein degradation rates for a total of 115 proteins could be estimated. Observed rates were, for example, at 0.056 and 0.049 h^-1 for phosphoglycerate kinase and enolase giving rise to the most stable signals."
Entered by Uri M
ID 110438