||Bacteria Escherichia coli
||Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BØ. A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information. Mol Syst Biol. 2007 3:121. doi:10.1038/msb4100155 p.7 right column 2nd paragraph, p.10 right column bottom paragraph, p.15 left column 7th paragraph & right column 2nd paragraphPubMed ID17593909
||"Using chemostat data for E. coli growing aerobically on
glucose (see Supplementary information), [researchers] estimated the
GAM [Growth associated
maintenance] and NGAM [non-Growth associated
maintenance] costs (Figure 3D). [They] found that an NGAM
value of 8.39 mmolATP/gDW/h [BNID 110422] and a GAM value of
59.81 mmolATP/gDW best fit the experimental data."
||"To determine the carbon, nitrogen, phosphorus and sulfur sources that
could support simulated growth, [researchers] screened all of the metabolites
that could be exchanged with the environment (i.e., exchange
reactions) in the iAF1260 and iJR904 models. The identified
metabolites formed the potential substrate sets (Table IV). Through
subsequent simulations, [researchers] set an arbitrary maximum flux of 20mmol
substrate/gDW/h for each potential substrate tested (consistent
with maximum observed substrate uptake rates in vivo) and optimized
for flux through the BOF(CORE) using Flux
balance analysis (FBA) and either iAF1260 or iJR904.
An OUR [oxygen uptake rate] of 18.5 mmol/gDW/h, the BOF(CORE), a NGAM of
8.39 mmolATP/gDW/h, a GAM of 59.81 mmolATP/gDW and no regulatory constrains were used during the growth condition analysis
of iAF1260 (for iJR904, see Reed et al, 2003)."