Molecular mass of cyclosporin synthetase

Range 650 - 800 kDa
Organism Fungus Tolypocladium inflatum (Beauveria nivea)
Reference Lawen A, Zocher R. Cyclosporin synthetase. The most complex peptide synthesizing multienzyme polypeptide so far described. J Biol Chem. 1990 Jul 5 265(19):11355-60. p.11360 left column 2nd paragraphPubMed ID2358465
Method "Measurements of the molecular weight of the native cyclosporin synthetase were performed by ultracentrifugation in glycerol gradients along with standard proteins."
Comments "The molecular mass of cyclosporin synthetase has been determined to be between 650 and 800 kDa. In spite of this high value, [researchers] were not able to dissociate the enzyme into subunits neither with urea nor with detergents like SDS nor with ß-mercaptoethanol."
Entered by Uri M
ID 110414