Ion-selective microelectrode measurements of pH and of K+, and Cl- concentrations in cells of open and closed stomatal complexes

Range Table - link %
Organism Asiatic dayflower Commelina communis
Reference Leigh R.A. (1997). Solute composition of vacuoles. In Advances in Botanical Research, Vol. 25: The Plant Vacuole, Leigh R.A., Sanders D., eds (London:Academic Press), pp. 171–194. p.178 table II
Primary Source Penny, M. G. and Bowling, D. J. F. (1975). Direct determination of pH in the stomatal complex of Commelina. Planta 122, 209-212. & Penny, M. G. and Bowling, D. J. F. (1974). A study of potassium gradients in the epidermis of intact leaves of Commelinii communis L. in relation to stomatal opening. Planta 119, 17-25. & Penny, M. G., Kelday, L. S. and Bowling, D. J. F. (1976). Active chloride transport in the leaf epidermis of Commelina communis in relation to stomatal activity. Planta 130, 291-294PubMed ID24435970, 24442405, 24424642
Method Ion-selective microelectrode measurements
Comments "[Ion-selective microelectrode measurements] are useful because they provide a direct determination of activity which is more thermodynamically relevant than concentration and, by necessity, the results are from individual cells. The value of this approach is exemplified by the work of Penny and Bowling (primary sources), who used different types of ion-selective electrodes to measure the changes in ion concentrations in different cells of the stomatal complex of Commelina communis during opening and closing. The results (Table II) show that there are large differences in pH and K+ and CI- concentrations in different cell types in the complex and that the directions of the ion gradients between different cells are reversed during opening and closing of the stomatal pore."
Entered by Uri M
ID 110399