Intracellular steady state concentrations of E. coli K12

Range Table - link nmol/liter
Organism Bacteria Escherichia coli
Reference Hiller J, Franco-Lara E, Weuster-Botz D. Metabolic profiling of Escherichia coli cultivations: evaluation of extraction and metabolite analysis procedures. Biotechnol Lett. 2007 Aug29(8):1169-78. p.1175 table 2PubMed ID17479221
Primary Source Buchholz A, Takors R, Wandrey C (2001) Quantification of intracellular metabolites in Escherichia coli K12 using liquid chromatographic-electrospray ionization tandem mass spectrometric techniques. Anal Biochem 295: 129–137 & Chassagnole C, Noisommit-Rizzi N, Schmid JW, Mauch K, Reuss M (2002) Dynamic modeling of the central carbon metabolism of Escherichia coli. Biotechnol Bioeng 79(1):53–73 & Schaub J, Schiesling C, Reuss M, Dauner M (2006) Integrated sampling procedure for metabolome analysis. Biotechnol Prog 22: 1434–1442PubMed ID11488613, 17590932, 17022684
Method This work-Liquid chromatography–mass spectrometry (LCMS). Other sources see primary sources
Comments "To validate the proposed sampling, quenching and extraction procedure, all investigated intracellular metabolite concentrations are compared with data from literature. The results are summarised in Table 2."
Entered by Uri M
ID 110378