Reference 
Mariani, L., Schulz, E. G., Lexberg, M. H., Helmstetter, C., Radbruch, A., Löhning, M., & Höfer, T. (2010). Shortterm memory in gene induction reveals the regulatory principle behind stochastic IL4 expression. Molecular systems biology, 6(1). p.12 table IPubMed ID20393579

Comments 
P.11 right column 2nd paragraph: "To obtain the number of IL‐4 protein produced per activated cell in 1 h, [investigators] calculated the increment during hours 3 and 4 (mean and s.d. are presented in Table I). To estimate the mean mRNA (relative to HPRT), [they] used the data from RT–PCR at hour 2, 2.5, and 3 (peak of IL‐4 mRNA production) after restimulation (three independent experiments). The relative level per producing cell was found by rescaling to the fraction of IL‐4 positive cells at the indicated time (mean and s.d. are presented in Table I). To estimate the absolute transcription rate in a producing cell (no/min), the mean mRNA level was divided by the lifetime of mRNA and multiplied by the mean number of HPRT molecules per cell, assumed to be equal to 20. To estimate the absolute translation rate in a producing cell (no/mRNA/min), [they] divided the estimated mean protein production per hour by the estimated mean number of mRNA molecules." 