Number of several spindle pole body proteins per cell

Range Arpc1p 64,700: Arp2p 78,100: Fim1p 90,700: Hxk1p 106,000: Gpd3p 546,000: Hxk2p 680,000 molecules/cell
Organism Fission yeast Schizosaccharomyces pombe
Reference McCormick CD et al., Measuring affinities of fission yeast spindle pole body proteins in live cells across the cell cycle. Biophys J. 2013 Sep 17 105(6):1324-35. doi: 10.1016/j.bpj.2013.08.017. Supporting information p.S9 Fig.S1DPubMed ID24047983
Method "Extension of the calibration curve for counting highly expressed mYFP fusion proteins in fission yeast. Plots of mean cellular fluorescence intensity vs. mean numbers of mYFP fusion proteins calculated from quantitative immunoblots of known numbers of whole cells solubilized in SDS and separated by SDS-PAGE."
Comments Standard deviations are (respectively) ±32,700, ±7,500, ±14,800, ±18,900, ±65,300, ±166,000
Entered by Uri M
ID 110102