Maturation time (τ) of newly synthesized RNAPs

Value 3.4 min
Organism Bacteria Escherichia coli
Reference Klumpp S, Hwa T. Growth-rate-dependent partitioning of RNA polymerases in bacteria. Proc Natl Acad Sci U S A. 2008 Dec 23 105(51):20245-50. doi: 10.1073/pnas.0804953105. p.20247 left column bottom paragraphPubMed ID19073937
Comments "Most parameters of [researchers] model have been measured (see ref. 4, SI Text, and Tables S1 and S2 BNID 110068, 110069), but the model contains 2 unknown parameters, the dissociation constant for nonspecific RNAP-DNA binding, Kns, and the maturation time t of newly synthesized RNAPs. [They] assume these parameters are independent of the growth rate themselves and determine them by matching the fraction of cytoplasmic RNAPs predicted by [their] model to data from minicell experiments (12, 13) (see SI Text and Fig. S1). This procedure leads to a maturation time t of 3.4 min and a dissociation constant for nonspecific binding of 3,100µM (BNID 110064). These values are consistent with experimental data..."
Entered by Uri M
ID 110063