Diffusion rate of transcription factor

Range 1D diffusion rate (along DNA) ~0.05: 3D diffusion rate (cytoplasmic) ~3 μm^2/sec
Organism Unspecified
Reference Li GW, Xie XS. Central dogma at the single-molecule level in living cells. Nature. 2011 Jul 20 475(7356):308-15. doi: 10.1038/nature10315. p.311 left column bottom paragraphPubMed ID21776076
Primary Source [50] Schurr JM. The one-dimensional diffusion coefficient of proteins absorbed on DNA. Hydrodynamic considerations. Biophys Chem. 1979 May9(4):413-4 [51] Blainey, P. C. et al. Nonspecifically bound proteins spin while diffusing along DNA. Nature Struct. Mol. Biol. 16, 1224–1229 (2009) doi: 10.1038/nsmb.1716PubMed ID380674, 19898474
Method P.311 left column bottom paragraph: "In a series of single-molecule studies in vitro, 1D diffusion has been directly observed for fluorescently labelled transcription factors and other DNA-binding proteins along nonspecific DNA under a microscope [refs 44-49]."
Comments P.311 left column bottom paragraph: "The observed 1D diffusion rate (of the order of 0.05µm^2/s) is much slower than the 3D diffusion in a living cell (~3µm^2/s) because the 1D diffusion of the transcription factor is coupled to simultaneous rotation around the DNA, tracking the pitch of the DNA double helix [primary sources]."
Entered by Uri M
ID 109954