Affinity (Km) at which acetyl-CoA synthetase (ACS) scavenges acetate

Value 200 μM
Organism Bacteria Escherichia coli
Reference Valgepea K, Adamberg K, Nahku R, Lahtvee PJ, Arike L, Vilu R. Systems biology approach reveals that overflow metabolism of acetate in Escherichia coli is triggered by carbon catabolite repression of acetyl-CoA synthetase. BMC Syst Biol. 2010 Dec 1 4: 166. doi: 10.1186/1752-0509-4-166. p.2 left column top paragraphPubMed ID21122111
Primary Source Kumari S, Beatty CM, Browning DF, Busby SJ, Simel EJ, Hovel-Miner G, Wolfe AJ. Regulation of acetyl coenzyme A synthetase in Escherichia coli. J Bacteriol. 2000 Aug182(15):4173-9.PubMed ID10894724
Comments "Acetic acid can be metabolized to acetyl-CoA either by the PTA-ACKA pathway or by acetyl-CoA synthetase (ACS) through an intermediate acetyl-AMP. The high affinity (Km of 200 µM for acetic acid) ACS scavenges acetate at low concentrations whereas the low affinity PTA-ACKA pathway (Km of 7-10 mM) is activated in the presence of high acetate concentrations [primary source]."
Entered by Uri M
ID 109945