Glucose concentration in derepressed cells

Range ~1.5 Table - link mM
Organism Budding yeast Saccharomyces cerevisiae
Reference Teusink B, Diderich JA, Westerhoff HV, van Dam K, Walsh MC. Intracellular glucose concentration in derepressed yeast cells consuming glucose is high enough to reduce the glucose transport rate by 50%. J Bacteriol. 1998 Feb180(3):556-62. abstract and p.559 table 1PubMed ID9457857
Method Abstract: "To determine if this regulation of glucose transport might be a consequence of intracellular free glucose [investigators] developed a new method to measure intracellular glucose concentrations in cells metabolizing glucose, which compares glucose stereoisomers to correct for adhering glucose."
Comments Abstract: "The intracellular glucose concentration was 1.5 mM, much higher than in most earlier reports." P.558 right column 2nd paragraph: "In the case of the derepressed cells described above, the calculated glucose consumption rate was very similar to the measured rate. Clearly, in derepressed cells consuming glucose, the intracellular glucose concentration is of sufficient magnitude to reduce the flux through the carrier by 50% (see Table 1). Further, the high-affinity transport system present in these cells is close to saturation at 13.3 and 34 mM extracellular glucose so it is not surprising that both the glucose consumption rates and the intracellular glucose concentrations were similar at these extracellular glucose concentrations (Table 1). Carryover of extracellular glucose, however, should differ greatly between these two cases, strongly suggesting that [investigators'] correction for carryover was effective."
Entered by Uri M
ID 109804