Genome size

Value 39936 bp
Organism Bacteriophage T7
Reference Dunn JJ, Studier FW. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J Mol Biol. 1983 Jun 5 166(4):477-535. p.477 abstractPubMed ID6864790
Method "The nucleotide sequence was determined on DNA from purified phage particles of wildtype T7 (Studier, 1969,1979) by the methods of Maxam & Gilbert (Maxam, A. M. & Gilbert, W. (1977). Proc. Nat. Acad. Sci., U.S.A. 74, 560-564. Maxam, A. M. & Gilbert, W. (1979). In Methods in Enzymology (Grossman, L. & Moldave, K., eds), vol. 65, pp. 499-559, Academic Press, New York.), using previously determined restriction maps of T7 DNA as the starting point for isolating specific DNA fragments (McDonell etal., 1977 Rosenberg etal., 1979 unpublished results). Computer programs for storing, searching and analyzing the nucleotide sequence were developed and applied by K. Thompson and W. Crockett."
Comments "The complete nucleotide sequence of bacteriophage T7 DNA, 39,936 base-pairs, has been determined by the techniques of Maxam & Gilbert."
Entered by Uri M
ID 109732