Cell concentration for culture with OD600=15 and OD600=30

Range 5.5 for OD600=15: 9.6 for OD600=30 gCDW/liter
Organism Bacteria Corynebacterium glutamicum
Reference Noack S, Nöh K, Moch M, Oldiges M, Wiechert W. Stationary versus non-stationary (13)C-MFA: a comparison using a consistent dataset. J Biotechnol. 2011 Jul 10 154(2-3):179-90. doi: 10.1016/j.jbiotec.2010.07.008. p.181 left column top paragraphPubMed ID20638432
Method "...an isotopically non-stationary fedbatch experiment was carried out for a C. glutamicum L-lysine producer. Labeling transients of metabolic intermediates were monitored until they reach equilibrated labeling states. LC–MS analytics is applied for measuring mass isotopomers of intermediates from the central metabolism of C. glutamicum."
Comments "For providing quantitative data of intracellular metabolite pool sizes samples were taken at OD600 = 15 (5.5 gCDW/l) and OD600 = 30 (9.6 gCDW/l), each in 5 technical replicates."
Entered by Uri M
ID 109702