Mean actin polymer length

Value 200 nm
Organism Amoeba Dictyostelium discoideum
Reference D.N. Robinson, Y.-S. Kee, T. Luo and A. Surcel, Understanding How Dividing Cells Change Shape. In: Edward H. Egelman, editor: Comprehensive Biophysics, Vol 7, Cell Biophysics, Denis Wirtz. Oxford: Academic Press, 2012. pp. 48-72. p.52 right column bottom paragraph
Primary Source Podolski, J. L. Steck, T. L. Length distribution of F-actin in Dictyostelium discoideum. J. Biol. Chem. 1990, 265, 1312–1318.PubMed ID2295631
Method A DNase I inhibition assay
Comments "Using a DNase I inhibition assay, Podolski and Steck determined that the mean polymer length is 200 nm, though they observe that 70% of the polymers are less than 140 nm (primary source). In spectacular agreement, the measured length distribution in the cleavage furrow was found to be 94 nm with a mean + 1SD of 140 nm (~80% of the polymers should be less than the mean +1SD, (ref 48, PMID 18372178) (Figure 3(a))."
Entered by Uri M
ID 109567