| Value |
200
nm
|
| Organism |
Amoeba Dictyostelium discoideum |
| Reference |
D.N. Robinson, Y.-S. Kee, T. Luo and A. Surcel, Understanding How Dividing Cells Change Shape. In: Edward
H. Egelman, editor: Comprehensive Biophysics, Vol 7, Cell Biophysics, Denis Wirtz. Oxford: Academic Press, 2012.
pp. 48-72. p.52 right column bottom paragraph |
| Primary Source |
Podolski, J. L. Steck, T. L. Length distribution of F-actin in Dictyostelium discoideum. J. Biol. Chem. 1990, 265, 1312–1318.PubMed ID2295631
|
| Method |
A DNase I inhibition assay |
| Comments |
"Using a DNase I inhibition assay, Podolski and Steck determined
that the mean polymer length is 200 nm, though they
observe that 70% of the polymers are less than 140 nm (primary source). In spectacular agreement, the measured length distribution in the
cleavage furrow was found to be 94 nm with a mean + 1SD of 140 nm (~80% of the polymers should be less than
the mean +1SD, (ref 48, PMID 18372178) (Figure 3(a))." |
| Entered by |
Uri M |
| ID |
109567 |