Cell cycle duration of HeLa cell

Range ~22 Hours
Organism Human Homo sapiens
Reference Posakony JW, England JM, Attardi G. Mitochondrial growth and division during the cell cycle in HeLa cells. J Cell Biol. 1977 Aug74(2):468-91. p.473 left column 5th paragraphPubMed ID885911
Method P.469 right column 3rd paragraph: "HeLa cells (strain F-315) were grown at 37°C (~22 h generation time) in roller bottles containing modified Eagle's medium (ref 11) plus 10% calf serum. Physiologically synchronized cell populations were produced by the selective detachment of mitotic cells from their substrate (refs 36, 44)."
Comments p.473 left column 5th paragraph: "Cell Cycle Parameters: Nuclear DNA synthesis and mitotic activity followed similar time-courses in the synchronous cultures derived from the three separate cell detachments (Fig. 3a). At 37˚ the cell cycle was found to be ~22 h in duration (as estimated from the second peak of mitotic activity), with the maximum of nuclear DNA synthesis at 15-16 h. The culture samples taken for morphometric analysis (at 0, 3, 7, 11, 16, and 19 h after the plating of mitotic cells, see Fig. 3a), after cell selection by the criteria described above (Materials and Methods), provided early, middle, and late "G1" cell populations (3, 7, and 11 h), an "S" population (16 h), a "G2" population (19 h), and an "M" population (0 h)."
Entered by Uri M
ID 109393