Turnover number for chorismate mutase

Value 50 Sec^-1
Organism Bacteria Bacillus subtilis
Reference Mattei P, Kast P, Hilvert D. Bacillus subtilis chorismate mutase is partially diffusion-controlled. Eur J Biochem. 1999 Apr261(1):25-32. p.26 left column top paragraphPubMed ID10103029
Primary Source Gray JV, Eren D, Knowles JR. Monofunctional chorismate mutase from Bacillus subtilis: kinetic and 13C NMR studies on the interactions of the enzyme with its ligands. Biochemistry. 1990 Sep 18 29(37):8872-8.PubMed ID2125470
Comments "In the case of the structurally distinctive BsCM, product dissociation has been proposed to be limiting under saturating conditions due to the coincidence of the rate constant for the dissociation of prephenate obtained from line-broadening experiments (~60s^-1 at 30°C) and the turnover number for the enzyme determined kinetically (50 s^-1) [primary source]."
Entered by Uri M
ID 109299