Turnover rate of purified HisQMP2 for hydrolysis of ATP

Value 8 1/sec
Organism Bacteria Salmonella typhimurium
Reference Liu CE, Liu PQ, Ames GF. Characterization of the adenosine triphosphatase activity of the periplasmic histidine permease, a traffic ATPase (ABC transporter). J Biol Chem. 1997 Aug 29 272(35):21883-91. p.21890 left column 2nd paragraphPubMed ID9268321
Method "ATP Hydrolysis Assay—Membrane vesicles and PLS were prepared as described earlier (ref 6), from strain TA1889 unless specified differently."
Comments "Purified HisQMP2 hydrolyzes ATP with a Vmax of 2100 nmol/min/mg of protein (Fig. 4), which is calculated to correspond to a turnover rate of 8 s^-1 in the presence of saturating liganded HisJ."
Entered by Uri M
ID 109032