Value |
8
1/sec
|
Organism |
Bacteria Salmonella typhimurium |
Reference |
Liu CE, Liu PQ, Ames GF. Characterization of the adenosine triphosphatase activity of the periplasmic histidine permease, a traffic ATPase (ABC transporter). J Biol Chem. 1997 Aug 29 272(35):21883-91. p.21890 left column 2nd paragraphPubMed ID9268321
|
Method |
"ATP Hydrolysis Assay—Membrane vesicles and PLS were prepared
as described earlier (ref 6), from strain TA1889 unless specified differently." |
Comments |
"Purified
HisQMP2 hydrolyzes ATP with a Vmax of 2100 nmol/min/mg of
protein (Fig. 4), which is calculated to correspond to a turnover
rate of 8 s^-1 in the presence of saturating liganded HisJ." |
Entered by |
Uri M |
ID |
109032 |