Normalized extracellular aqueous volume (Vex) for E. coli BL21(DE3)

Value 3.6 μL/mg
Organism Bacteria Escherichia coli
Reference Wang, Lei, Zhou, Yongjin J., Ji, Debin, Zhao, Zongbao K., An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells, Journal of Microbiological Methods (2013), doi: 10.1016/j.mimet.2013.02.006 p.7 top paragraphPubMed ID23481146
Method Determining the Vex value by using NAD+ as the probe
Comments "Vex values for E. coli BL21(DE3) and A. tumefaciens AGL1 were estimated to be 45.3 µL and 168.3 µL, respectively. Because DCW of E. coli BL21(DE3) and A. tumefaciens AGL1 were determined to be 12.7 mg and 45.3 mg, the normalized Vex values were 3.6 µL·mg–1 and 3.7 µL·mg–1, respectively."
Entered by Uri M
ID 108811