Yield coefficients for the anaerobic growth of E. coli on glucose

Range Table - link
Organism Bacteria Escherichia coli
Reference Karel SF, Robertson CR. Cell mass synthesis and degradation by immobilized Escherichia coli. Biotechnol Bioeng. 1989 Jul34(3):337-56. p.341 table IIPubMed ID18588111
Method Abstract: "Escherichia coli K-12 cells were grown in a confined volume using microporous hollow fiber membranes. The local cell concentrations in the reactors were above 400 g dry mass/L, in excess of the predicted limit based on the specific volume of free cells determined by tracer exclusion. Cell mass synthesis and degradation rates in these reactors were measured using radioisotope labeling with (35)S. Net accumulation of cell material persisted at these high cell densities. The rates of substrate uptake and cell growth were predicted from the theory of reaction and diffusion assuming that kinetics of cell metabolism are identical for free-living and immobilized cells. This theory was tested by comparison of overall rates and by the size of the region in which cell growth occurred, measured by autoradiography. A yield coefficient of 4 +/- 1 mol sulfur/mol glucose was measured, in agreement with the value determined for free-living cells in similar conditions."
Comments P.340 right column bottom paragraph: "The concentration of (35)S incorporated in the cells increases in parallel with other measures of the cell mass during the growth phase, and is in fact linearly related to protein or cell mass synthesis. Yield coefficients calculated from linear regressions of various measures of cell, substrate, and products concentrations derived from batch experiments are summarized in Table II." P.343 right column bottom paragraph: "The ratio of H+ production to glucose consumption rates Y(H/G), was determined for reactors III (2.2±O.1) and IV (2.4±0.6). These ratios agree quite well with Y(H/G) determined for free cells (Table II)." See notes beneath table
Entered by Uri M
ID 108658