| Value |
42
nts/sec
Range: Table - link nts/sec
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.505 table 1PubMed ID20413502
|
| Method |
"To calculate the elongation rate, the
Escherichia coli culture was induced with IPTG,
and the time that elapsed between the appearance
of a specific hybridization signal from
probes complementary to the 5' and 3' segments
of the lacZ transcript was determined by dotblot
hybridization (Fig. 1 and fig. S1) (5, 6)." See note above table |
| Comments |
"In the absence of Cm (chloramphenicol), the
transcription elongation rate was determined to
be 42 nucleotides (nt) per second under specified
growth conditions, matching a translation
elongation rate of 14 amino acids per second
(Table 1 and fig. S2)." Strain MG1655 |
| Entered by |
Uri M |
| ID |
108488 |