Cytosolic protein content determined by mass spectrometry

Value 107 g/liter
Organism Bacteria Mycoplasma pneumoniae
Reference Maier T, Schmidt A, Güell M, Kühner S, Gavin AC, Aebersold R, Serrano L. Quantification of mRNA and protein and integration with protein turnover in a bacterium. Mol Syst Biol. 2011 Jul 19 7: 511. doi: 10.1038/msb.2011.38. Supplementary material p.4 bottom line equation 4PubMed ID21772259
Method "...the total cellular protein content was determined by summing up the determined individual cellular protein masses (5.34 fg or 3.22 GDa). Dividing the thus determined average cellular protein content by the average cell volume V_cell (0.05 µm^3) resulted in a mass spectrometry based determination of the cytosolic protein concentration C_cytosolMS."
Entered by Uri M
ID 108344