pH of vacuole

Value 6.17 unitless
Organism Budding yeast Saccharomyces cerevisiae
Reference Preston RA, Murphy RF, Jones EW. Assay of vacuolar pH in yeast and identification of acidification-defective mutants. Proc Natl Acad Sci U S A. 1989 Sep86(18):7027-31. p.7029 right column 2nd paragraphPubMed ID2674942
Method "...[Researchers] developed assays of vacuolar pH using flow cytometry and fluorescence microscopy, based on the pH-dependent fluorescence of 6-carboxyfluorescein (6-CF)."
Comments "Cytometric analysis of wild-type cells labeled with 6-CF showed that vacuolar pH was maintained at 6.17 (SD = 0.07, n = 16) under several different conditions tested." pH of vacuole varies from <5 to 6.5 according to Li et al., 2009 PMID 18786576 p.654 left column bottom paragraph
Entered by Uri M
ID 108318